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KMID : 0043320060290080645
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Archives of Pharmacal Research
2006 Volume.29 No. 8 p.645 ~ p.650
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Effects of Tributyltin Chloride on L-DOPA-Induced Cytotoxicity in PC12 Cells
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Lee Jae-Joon
Kim Yu-Mi
Park Seung-Kook
Lee Myung-Koo
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Abstract
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Tributyltin chloride (TBTC) at concentrations of 0.5-1.0 μM inhibits dopamine biosynthesis in PC12 cells. In this study, the effects of TBTC on L-3,4-dihydroxyphenylalanine (L-DOPA)- induced cytotoxicity in PC12 cells were investigated. TBTC at concentrations up to 1.0 μM neither affected cell viability, nor induced apoptosis after 24 or 48 h in PC12 cells. However, TBTC at concentrations higher than 2.0 μM caused cytotoxicity through an apoptotic process. In addition, exposure of PC12 cells to non-cytotoxic (0.5 and 1.0 μM) or cytotoxic (2.0 μM) concentrations of TBTC in combination with L-DOPA (20, 50 and 100 μM) resulted in a significant increase in cell loss and the percentage of apoptotic cells after 24 or 48 h compared with TBTC or L-DOPA alone. The enhancing effects of TBTC on L-DOPA-induced cytotoxicity were concentration- and treatment time-dependent. These data demonstrate that TBTC enhances L-DOPA-induced cytotoxicity in PC 12 cells.
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KEYWORD
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Tributyltin chloride, L-3, 4-Dihydroxyphenylalanine (L-DOPA), Cytotoxicity, Apoptosis, PC12 cells
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